Electrophoretic Mobility Shift Assay: Analyzing Protein – Nucleic Acid Interactions
نویسندگان
چکیده
The characterization of protein-nucleic acid interactions is essential not only for understanding the wide range of cellular processes they are involved in, but also the mechanisms underlying numerous diseases associated with the breakdown of regulatory systems. These include, but are far from being limited to, cell cycle disorders such as cancer and those caused by pathogenic agents that rely on or interfere with host cell machinery. More recently, it has been hypothesized that many neurological disorders such as Alzheimer’s, Huntington’s, Parkinson’s, and polyglutamine tract expansion diseases are a consequence, at least in part, of aberrant protein-DNA interactions that may alter normal patterns of gene expression (Jiménez, 2010).
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