Two-deoxyglucose as an anti-metabolite in human carcinoma cell line RPMI-2650 and drug-resistant variants.
نویسندگان
چکیده
The accumulation of 2-deoxyglucose (2-DG), a glycolytic inhibitor, was investigated in a human nasal carcinoma cell line, RPMI-2650 and two of its drug-resistant variants (selected with taxol and melphalan) to assess manipulation of glycolytic potential as a selective means of reducing resistance. 2-DG uptake was increased 3-fold and 9.9-fold in taxol- and melphalan-resistant variants of RPMI-2650, respectively. Two of the principal factors associated with increased 2-DG uptake, namely glucose transporters and hexokinase activity, were increased in the resistant variants. Other changes in glucose metabolism that may affect 2-DG as an antimetabolite were observed, including increases in glucose-6-phosphate dehydrogenase of 10-fold and 100-fold for taxol- and melphalan-resistant variants, respectively, suggesting higher pentose phosphate activity; increased glutamine utilisation and greater sensitivity to iodoacetic acid-induced depletion of ATP levels in the parent relative to the resistant variants.
منابع مشابه
Effects of Over-Expression of LOC92912 Gene on Cell Cycle Progression
Background: We had previously identified the genes involved in squamous cell carcinoma of the head and neck using differential display and DNA microarray techniques. We also reported the first analytical study on a novel human gene called LOC92912, which was identified by differential display as a gene up-regulated in such carcinomas. LOC92912, which is a putative member of the E2 ubiquitin con...
متن کاملتأثیر آدنوزین ´5تری فسفات در القای آپوپتوز و مهار بیان ژن Survivin و واریانت پیرایشی ضد آپوپتوزی SUR-3B آن در سلول های K562
Introduction: Leukemia is a heterogeneous malignant disease in which progression at the level of CD34+ cells has a major impact in drug resistance and relapse. The multi-drug resistance gene product, P-glycoprotein is an inhibitor of apoptosis proteins (IAPs), such as Survivin that are expressed simultaneously with several putative drug resistance parameters in CD34+ leukemia cells. In fact, IA...
متن کاملParadoxical regulation of human argininosuccinate synthetase cDNA minigene in opposition to endogenous gene: evidence for intragenic control sequences.
Human somatic cell variants resistant to the arginine analog, canavanine, express 200-fold increased levels of argininosuccinate synthetase (AS) mRNA as compared to parental cells. In this study we examined whether AS cDNA sequences contain cis-acting regulatory elements that are involved in the induction of AS mRNA in canavanine-resistant cells. Minigene constructs containing AS cDNA sequences...
متن کاملRegulation of human argininosuccinate synthetase gene: induction by positive-acting nuclear mechanism in canavanine-resistant cell variants.
Nonhepatic human cell variants resistant to the arginine analog, canavanine, express argininosuccinate synthetase (AS) mRNA at levels 200-fold higher than parental cells without amplification of AS gene sequences. In this report we show that this regulation occurs in the nucleus prior to polyadenylation of AS precursor RNA and occurs through a positive-acting mechanism operating in canavanine-r...
متن کاملCytotoxic effect of curcumin on viability and P21 and Bcl-2 genes expression of the human anaplastic thyroid Carcinoma cells line (SW-1736)
Background & Aim: Anaplastic thyroid carcinoma (ATC) is an uncommon malignancy of the thyroid .with poor prognosis and 2 to 6 month of survival time. The aim of this study was to explore the effect of curcumin on bioavailability and expression of p21 and Bcl-2 genes in human anaplastic thyroid carcinoma cell line (SW-1736). Methods: In this study, human anaplastic thyroid carcinoma (SW-1736) c...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Anticancer research
دوره 24 2A شماره
صفحات -
تاریخ انتشار 2004