Development on ethanol production from xylose by recombinant Saccharomyces cerevisiae
نویسندگان
چکیده
Xylose is the second major fermentable sugar present in lignocellulosic hydrolysates, so its fermentation is essential for the economic conversion of lignocellulose to ethanol. However, the traditional ethanol production strain Saccharomyces cerevisiae does not naturally use xylose as a substrate. A number of different approaches have been used to engineer yeasts to reconstruct the gene background of S. cerevisiae in recent years. The recombinant strains showed better xylose fermentation quality by comparison with the natural strains. This review examines the research on S. cerevisiae strains that have been genetically modified or adapted to ferment xylose to ethanol from three aspects including construction of xylose transportation, xylose-metabolic pathway and inhibitor tolerance improvement of S. cerevisiae.
منابع مشابه
Optimal growth and ethanol production from xylose by recombinant Saccharomyces cerevisiae require moderate D-xylulokinase activity.
D-Xylulokinase (XK) is essential for the metabolism of D-xylose in yeasts. However, overexpression of genes for XK, such as the Pichia stipitis XYL3 gene and the Saccharomyces cerevisiae XKS gene, can inhibit growth of S. cerevisiae on xylose. We varied the copy number and promoter strength of XYL3 or XKS1 to see how XK activity can affect xylose metabolism in S. cerevisiae. The S. cerevisiae g...
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