Thiopental and isoflurane attenuate the decrease in hippocampal phosphorylated Focal Adhesion Kinase (ppFAK) content induced by oxygen–glucose deprivation
نویسنده
چکیده
Results. Significant neuronal death was detected after 30 min (but not 10) of anoxia-aglycaemia (40 (4) vs 14 (5)% of control, P<0.05). At 30 min, phosphorylated ppFAK content was significantly decreasedby anoxic glucose-free conditions (55 (27)%of control, P<0.05). This effectwasmarkedly attenuated by thiopental (10 and 100 mM) and isoflurane (1 and 2%). Under control conditions, thiopental (1, 10, and 100mM) and isoflurane (0.5, 1, and 2%) increased ppFAK phosphorylation in a concentration-related fashion.This effectwasblockedbychelerythrin andbisindolylmaleimide I and IX (10mM, three structurally distinct inhibitorsof protein kinase C, PKC) but not the N-methylD-aspartate (NMDA) receptor antagonist MK801 (10 mM).
منابع مشابه
Thiopental and isoflurane attenuate the decrease in hippocampal phosphorylated Focal Adhesion Kinase (pp125FAK) content induced by oxygen-glucose deprivation.
BACKGROUND Thiopental and isoflurane exhibit neuroprotective effects against cerebral ischaemia. Here, we hypothesized that oxygen-glucose deprivation decreases the ATP-dependent phosphorylation process of Focal Adhesion Kinase (pp125FAK, a functionally important non-receptor tyrosine kinase), and that this phenomenon is attenuated by thiopental and isoflurane. METHODS Rathippocampal slices w...
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