Effect of Salvia triloba L. f. Extracts on Neoplastic Cell lines

صخلملا The search for novel anticancer drugs continues. This work includes a preliminary study of the effect of two crude extracts of Greek or Mediterranean sage (Salvia triloba L. f.) on three malignant human cell lines and one malignant murine cell line. A boiling water extract and a methanolic extract were prepared from dried leaves of S. triloba. Yields of extraction were 9.8 and 22.4%, respectively. Tested cell lines included human larynx epidermoid carcinoma (HEp-2), human rhabdomyosarcoma (RD), human glioblastoma multiforme (AMGM5) and murine mammary adenocarcinoma (AMN3). Both extracts exhibited time-dependent, cell specific inhibitory effects on HEp-2, RD and AMN3 malignant cell lines. AMGM5 cell line was resistant to the effects of both extract as inhibition could only be recorded after 72 hrs of exposure to the highest extracts concentrations, 625 and 1250 μg/ml. In addition, growth of HEp-2, RD and AMN3 cells under treatment with either extract was biphasic during the first 48 hrs of treatment as cells were stimulated at lower concentrations and inhibited at higher concentrations. It seems likely that extracts of Salvia triloba may act at various therapeutic levels, especially in cancer treatment, but further research is required to evaluate the practical values of therapeutic application. ثحبلا أ نع هركتبم هيود ناطرسلا دض ِ ّ ّ ي َ ُّ رمتس . يفو اذھ انمق ثحبلا ب ةسارد تاصلختسملل لا طسوتملا ضيبلأا رحبلا ةيمريم نم ماخ َ ِ ِ ّ ْ ِّ وأ َ هيمريملا ينانويلا ه ) (Salvia triloba L.F. ىلع هعبرا طامنا لخ هيو ةثيبخ ِ . لوناثيملا و يلغملا ءاملا تاصلختسم تزھج ةففجملا قارولأا نم ِ ِ َّ َ ُ ِ ْ هيمريملل . تناك دقو صلاختسلإا هيمك 9.8 و 22.4 % يلاوتلا ىلع ، . تنمضت ْ ّ َ هيولخلا طامنلأا ةبرجملا ّ ُ طامنأ هعبرأ : ِ human larynx epidermoid carcinoma (HEp-2), human rhabdomyosarcoma (RD), human glioblastoma multiforme (AMGM5) and murine mammary adenocarcinoma (AMN3). تقولا ىلع ادمتعم ناك نيصلختسملا لاك لمع هقيرط ، ريثأتلا لدعمو ةثيبخلا هيولخلا طامنلأا ىلع صصختملا يولخلا يطيبثتلا HEp-2, RD , AMN3 احضاو ناك . يولخلا طمنلا امنيب AMGM5 امواقم ناك نم عون كانھ ناك نكلو نيصلختسملا لاكل يولخلا يطيبثتلا ريثأتلل ا طيبثتلا هدمل تاصلختسملل ضرعتلا دعب يولخل 72 زيكارتبو هعاس تغلب هيلاع 625 و / μg 1250 ml . ايلاخلا رثاكت ناك كلذل ةفاضلإاب HEp-2, RD, AMN3 ثيح روطلا يئانث نيصلختسملا لاك ريثأت تحت لوأ يف هليلقلا زيكارتلاب ايلاخلا تزفح 48 يف هطبثم تناكو ةعاس هيلاعلا زيكارتلا . دبي ْ َ نأ و ّ هيمريملا هتبن تاصلختسم ،هيعيبطلا ةيجلاعلا هيصاخلا يف ديفت ِ ّ ،ناطرسلا جلاع يف اصوصخ ِ ً ديدحتل بولطم ضيفتسملا ثحبلا نكلو َ َ يجلاعلا قيبطتلل ةيلمعلا ميقلا ِ ّ ِ ِ ِ ِ َ . © 2010 Jordan Journal of Biological Sciences. All rights reserved