Adsorption-Elution Techniques and Molecular Detection of Enteric Viruses from Water
ثبت نشده
چکیده
Submit Manuscript | http://medcraveonline.com in drinking water. Faecal coliforms are used as indicators because of the assumption that the fate and transport of faecal coliforms reflect that of the waterborne pathogens [1]. However, accumulating evidence on waterborne disease outbreaks shows that absence of faecal coliform indicators does not entirely eliminate possibilities of the presence of other pathogens with a potential to cause disease outbreaks [2]. For example some enteric viruses are resistant to many disinfectants and remain viable after disinfection [3]. Studies have also demonstrated that there is no relationship between current bacterial indicator detection and the presence of viruses in treated water [4]. There is therefore need to have multiple or supplementary indicators which include enteric viruses and if possible to directly monitor the levels of viral pathogens in surface waters, irrigation water, sewage effluent as well as treated drinking water for public health safety and quality assessment [5]. However due to the low concentration of viruses in water matrices and presence of inhibitors, efficient concentration methods from large quantities are essential [5,6].
منابع مشابه
Molecular Detection of Hepatitis A Virus in Urban Sewage in Isfahan, Iran
ABSTRACT Background and Objectives: Hepatitis A virus (HAV) enters sewage through the stool of infected patients and contaminates freshwater and seawater. The aim of present study was quantitative molecular detection of HAV in raw and treated urban sewage samples from Isfahan, center of Iran. &nbs...
متن کاملAttachment of enteric viruses to bottles.
Storage of water that was deliberately contaminated with enteric viruses in polyethylene terephthalate (PET) bottles led to a rapid decrease of the apparent viral load, thereby hampering the development of samples for a collaborative evaluation of viral detection methods for bottled water. To determine if this decrease was due to spontaneous inactivation or to adhesion, an elution protocol was ...
متن کاملEvaluation of electropositive filtration for recovering norovirus in water.
The virus adsorption-elution (VIRADEL) technique has been widely used in the recovery of various enteric viruses in water, and an electropositive filter such as 1 MDS has been commonly applied. However, effective methods of monitoring waterborne norovirus (NoV) have not yet been well characterized and optimized. Hence, in this study, the VIRADEL technique was evaluated and optimized for effecti...
متن کاملDetection of poliovirus, hepatitis A virus and rotavirus from sewage and water samples.
A modified adsorption-elution technique for concentration of enteric viruses from sewage and water samples was developed. The viruses in water were concentrated by negatively charged membrane filtration, eluted with 2.9% tryptose phosphate broth containing 6% glycine pH 9.0, and reconcentrated using centrifugation by a speedVac concentrator. The presence of poliovirus, hepatitis A virus (HAV) R...
متن کاملImmunoaffinity concentration and purification of waterborne enteric viruses for detection by reverse transcriptase PCR.
To assess the risks from viral contamination of drinking-water supplies, there is a clear need for methods to directly detect viral pathogens. In this study, we developed a broad-spectrum immunocapture method for concentration and purification of enteric viruses. The method involved indirect antibody capture (AbCap) of intact viruses followed by release of virion genomic RNA and reverse transcr...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2016