Koilocytes are enriched for alkaline-labile sites
نویسندگان
چکیده
This study investigated possible variations in the chromatin structure of koilocytes resulting from human papillomavirus (HPV) infection. Alkaline-labile sites (ALS) were detected with the DNA breakage detection–fluorescence in situ hybridization (DBD-FISH) technique using a whole human genome DNA probe obtained from individuals without koilocytosis. The variable levels of ALS present were measured quantitatively using image analysis after whole-genome DNA hybridization. A significant increase in the number of ALS was observed in koilocytes compared with normal cells. We demonstrated that the presence of ALS could be an indicator of chromatin change in koilocytes caused by HPV infection.
منابع مشابه
Alkaline lability of fluorescent photoproducts produced in ultraviolet light-irradiated DNA.
Ultraviolet light induces alkaline labile lesions in DNA. These lesions occur at the bipyrimidine sites T-C, C-C, and T-T, and do not result from the formation of pyrimidine cyclobutane dimers. To examine the chemical nature of the alkaline labile lesions, pyrimidine dinucleotides (2'-deoxythymidylyl-(3' leads to 5')-2'-deoxycytidine, 2'-deoxythymidylyl-(3' leads to 5')-2'-deoxythymidine, 2'-de...
متن کاملCobalt(III), a probe of metal binding sites of Escherichia coli alkaline phosphatase.
To facilitate the study of individual metal binding sites of polymeric metalloproteins, conversion of exchange-labile Co(II) in E. coli alkaline phosphatase (EC 3.1.3.1) to exchange-inert Co(III) was examined. Oxidation of Co(II) alkaline phosphatase with hydrogen peroxide results in a single absorption maximum at 530 nm and loss both of the characteristic electron paramagnetic signal and of en...
متن کاملDNA cross-linking and single-strand breaks induced by teratogenic concentrations of 4-hydroperoxycyclophosphamide and phosphoramide mustard in postimplantation rat embryos.
Postimplantation rat embryos (Day 10) were exposed in vitro to teratogenic concentrations of 4-hydroperoxycyclophosphamide, an activated form of cyclophosphamide, and phosphoramide mustard, the major teratogenic metabolite of cyclophosphamide. Following a 5-h exposure to these agents, drug-induced DNA damage was assessed by alkaline elution. Both drugs induced detectable DNA cross-linking at te...
متن کاملPreferential DNA repair of alkali-labile sites within the active insulin gene.
DNA damage and repair were studied in a DNA fragment containing the insulin gene after treatment of cells with methylnitrosourea. For these studies, two clonal isolates from the same rat insulinoma cell line which differ in that the insulin gene is transcribed in one (RINr 38) and is silent in the other (RINr B2) were utilized. Both the determination of immunologically reactive insulin released...
متن کاملBleomycin-induced alkaline-labile damage and direct strand breakage of PM2 DNA.
Bleomycin-induced breakage of an isolated covalently closed circular DMA from bacteriophage PM2 was as sayed fluorometrically after agarose gel electrophoresis and staining with ethidium bromide. When bleomycindamaged DMA was assayed under neutral conditions, there was a decrease in the amount of Form I DMA and a simultaneous increase in both Forms II and III of DMA. However, when the damage wa...
متن کامل