?Revival of Spermatozoa after Dehydration and Vitrification at Low Temperatures? (1949), by Christopher Polge, Audrey Ursula Smith, and Alan Sterling Parkes
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چکیده
In the 1949 article ?Revival of Spermatozoa after Dehydration and Vitrification at Low Temperatures,? researchers Christopher Polge, Audrey Ursula Smith, and Alan Sterling Parkes demonstrated that glycerol prevents cells from dying while being frozen. Polge and his colleagues discussed several procedures in which they had treated sperm [4] cells from various species with glycerol, froze those cells, and then observed the physiological effects that freezing had on the treated sperm [4]. The researchers concluded that glycerol safely preserves sperm [4] samples from a variety of species. Polge, Smith, and Parkes?s 1949 article detailed one of the first successful uses of a chemical medium to preserve viable [5] cells in a frozen state, a process that eventually enabled the first vertebrate embryo to be successfully conceived using frozen sperm [4].
منابع مشابه
The immobilization of spermatozoa by freezing and thawing and the protective action of glycerol.
It was discovered by Polge, Smith & Parkes (1949) that spermatozoa could be frozen and thawed without loss of motility if glycerol was included in their suspending medium. Following this discovery the spermatozoa of the domestic fowl (Polge, 1951), ofthe bull (Polge & Rowson, 1952) and ofthe herring (Blaxter, 1953) have been preserved without loss of function for long periods in the frozen stat...
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Twentieth-century researcher Ernest John Christopher Polge studied the reproductive processes of livestock and determined a method to successfully freeze, thaw, and utilize viable [4] sperm [5] cells to produce offspring in animals. In 1949, Polge identified glycerol as a cryoprotectant, or a medium that enables cells to freeze without damaging their cellular components or functions. Several ye...
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HE initial discovery by POLGE, SMITH and PARKES (1949) that glycerol aff ords protection to spermatozoa during freezing and thawing subsequently led to the development of semen banks which have greatly facilitated livestock improvement especially in the cattle breeding industry. However, until recently, the few attempts to preserve mammalian embryos by freezing have been relatively unsuccessful...
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SINCE it was discovered by Polge, Smith, and Parkes (1949) that living cells could be protected during freezing and thawing by adding glycerol to the storage medium, various substances and methods have been used in an attempt to achieve better results (Fleming,: 1952; Eastcott, Cross, Leigh, and North, 1954; Lovelock, 1954). We have been concerned with retaining the viability of corneal tissue ...
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The cryopreservation of organs became an active area of research in the 1950s as a result of the rediscovery of the cryoprotective properties of glycerol by Polge, Smith, and Parkes in 1949. Over the ensuing four decades of research in this area, the advantages of vitrification, or ice-free cryopreservation, have become apparent. To date, experimental attempts to apply vitrification methods to ...
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