21-P021 Efficient gene transfer to developing chick astrocytes by Tol2 transposition
نویسندگان
چکیده
pulmonary epithelial cell types. Additionally, by RNA microarray-analysis of laser-microdissected intrapulmonary epithelium we identified 218 potential target genes which were affected either by loss of-Dll1, or after Notch-inhibition, or under both conditions. These genes are currently being evaluated in silico as well as by expression analysis in wildtype and Notch signaling mutant embryos.
منابع مشابه
21-P025 Transcriptional regulation of GABAergic neuron development in the developing midbrain
studying vertebrate development, since the embryo could be a favorable target for gene transfer by in ovo electroporation in addition to classical manipulative advantages. However, it is still difficult to deliver transgenes into specific tissues at later developmental period, because common transfection methods are transient. We show here efficient transfer of EGFP driven by the GFAP promoter/...
متن کامل21-P024 Ptf1a enforces an inhibitory fate upon glutamatergic lineages in the zebrafish retina
studying vertebrate development, since the embryo could be a favorable target for gene transfer by in ovo electroporation in addition to classical manipulative advantages. However, it is still difficult to deliver transgenes into specific tissues at later developmental period, because common transfection methods are transient. We show here efficient transfer of EGFP driven by the GFAP promoter/...
متن کامل21-P022 Repression of the Notch signaling activity via Mesp2 is essential for somitogenesis
studying vertebrate development, since the embryo could be a favorable target for gene transfer by in ovo electroporation in addition to classical manipulative advantages. However, it is still difficult to deliver transgenes into specific tissues at later developmental period, because common transfection methods are transient. We show here efficient transfer of EGFP driven by the GFAP promoter/...
متن کاملTransposition of the Tol2 element, an Ac-like element from the Japanese medaka fish Oryzias latipes, in mouse embryonic stem cells.
The Tol2 transposable element of the Japanese medaka fish belongs to the hAT family of transposons including hobo of Drosophila, Ac of maize, and Tam3 of snapdragon. To date, Tol2 is the only natural transposon in vertebrates that has ever been shown to encode a fully functional transposase. It has not been known, however, whether Tol2 can transpose in vertebrates other than fish. We report her...
متن کاملFunctional dissection of the Tol2 transposable element identified the minimal cis-sequence and a highly repetitive sequence in the subterminal region essential for transposition.
The Tol2 element is a naturally occurring active transposable element found in vertebrate genomes. The Tol2 transposon system has been shown to be active from fish to mammals and considered to be a useful gene transfer vector in vertebrates. However, cis-sequences essential for transposition have not been characterized. Here we report the characterization of the minimal cis-sequence of the Tol2...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Mechanisms of Development
دوره 126 شماره
صفحات -
تاریخ انتشار 2009