Improved Method for the Isolation of Biosurfactant Glycolipids from Rhodococcus sp. Strain H13A.

نویسنده

  • F O Bryant
چکیده

An improved method for the isolation of the biosurfactant glycolipids from Rhodococcus sp. strain H13A by using XM 50 diafiltration and isopropanol precipitation was devised. This procedure was advantageous since it removes protein coisolated when the glycolipids are obtained by organic extraction and silicic acid chromatography. The protein apparently does not contribute any biosurfactant characteristics to the glycolipids. The deacylated glycolipid backbone included only a disaccharide.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Isolation and Identification of a Sulfide/Sulfoxide Monooxygenase Gene from a Newly Isolated Rhodococcus Sp. Strain FMF

Rhodococcus FMF is a gram-positive bacterium isolated for the first time from soil samples of Tabriz refinery in Iran. This microorganism is able to catabolize dibenzothiophene to 2-hydroxybiphenyl and inorganic sulfur without the destruction of carbon-carbon bonds. Three structural genes, dszA, dszB, and dszC have been characterized and shown to be responsible for this phenotype. In this work,...

متن کامل

Extracellular Cholesterol Oxidase from Rhodococcus sp.: Isolation and Molecular Characterization

Background: Cholesterol oxidase (CHO) has various clinical and industrial applications. Recently, microbial CHO have received a great attention for their wide usage in medicine. Here, taxonomic characterizations of isolated strain from soil, optimization of the conditions for CHO production and biochemical characterizations of produced CHO enzyme were described. Finally, CHO gene was cloned int...

متن کامل

Genome Sequence of Rhodococcus sp. Strain PML026, a Trehalolipid Biosurfactant Producer and Biodegrader of Oil and Alkanes

Rhodococcus sp. strain PML026 produces an array of trehalolipid biosurfactant compounds in order to utilize hydrophobic carbon sources, such as oils and alkanes. Here, we report the high-quality draft genome sequence of this strain, which has a total length of 5,168,404 bp containing 4,835 protein-coding sequences, 12 rRNAs, and 45 tRNAs.

متن کامل

Diesel Oil Utilization Efficiency of Selective Bacterial Isolates from Automobile Workshop and Thesjaswini River of Kerala

Bioremoval and bioreduction activities of hydrocarbon (diesel) isolation from environmental samples were studied by the activity of biosurfactant production, and calculating emulsification index, gravimetric, and FTIR analysis along with the estimation of bacterial biomass. Sample from soil near petrol, diesel pumps and water sample from Thesjaswini River near Padannakad, Kasaragod, Kerala, Ind...

متن کامل

Diesel Oil Utilization Efficiency of Selective Bacterial Isolates from Automobile Workshop and Thesjaswini River of Kerala

Bioremoval and bioreduction activities of hydrocarbon (diesel) isolation from environmental samples were studied by the activity of biosurfactant production, and calculating emulsification index, gravimetric, and FTIR analysis along with the estimation of bacterial biomass. Sample from soil near petrol, diesel pumps and water sample from Thesjaswini River near Padannakad, Kasaragod, Kerala, Ind...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Applied and environmental microbiology

دوره 56 5  شماره 

صفحات  -

تاریخ انتشار 1990