Complement fixation antibody test for human nocardiosis.

Anticomplement immunofluorescence test that uses isolated fibroblast nuclei for detection of antibodies to human cytomegalovirus.

Cytomegalovirus antibodies were measured in human sera by a nuclear anticomplement immunofluorescence test that used as antigen the isolated nucleic of virus-infected fibroblasts cells lysed in distilled water. The method exhibited less nonspecific fluorescence than either a conventional whole-cell anticomplement immunofluorescence test or an indirect fluorescent antibody test applied to the sa...

Correlation of herpes simplex virus antibody titers and specific lymphocyte stimulation in adult blood donors.

Antibody titers to herpes simplex virus type 1 in sera from healthy adult donors were assayed by complement fixation, microneutralization, and an enzyme immunoassay (ELISA). This last test proved to be the most sensitive method for antibody detection. It was estimated that ELISA antibody titers were up to 40-fold higher than neutralizing antibody titers and up to 100-fold higher than complement...

Detection of rotavirus by Latex Agglutination Test (Rotales); Comparison with Electron Microscopy and Complement Fixation Test

Estimation and identification of Streptococcus dysgalactiae protective antibody in sera of rabbits and cattle.

Rabbit and cow anti-Streptococcus dysgalactiae sera were tested by bacterial agglutination, complement fixation, hemagglutination, and immunodiffusion for the presence of antibody. The results of these tests were compared with mouse-protection studies on the same serum to estimate which in vitro test would best reflect the in vivo protective capacity of serum. Identification of the antibody con...

Clinical evaluation of a complement fixation test using human hydatid cyst fluid antigen for the detection of Echinococcus granulosus antibody

Complement fixation (CF) is a commonly used serodiagnostic test for hydatid disease, especially recommended for the evaluation of cure after surgery or for checking on treatment with antihelminthic drugs (9). However, the absence of an FDA-approved standardized antigen makes it difficult to assess the performance of different CF tests by using published studies. The objectives of this study wer...