Biological Studies on Capsulated Yeasts.
نویسندگان
چکیده
The taxonomic nomenclature of Stelling-Dekker (1931) and Lodder (1934) is used throughout the paper. The yeast strains were derived partly from the stock of the Department of Bacteriology and Hygiene of the Hebrew University and partly from the following type culture collections: National Type Culture Collection, London; American Type Culture Collection, Washington; and the Centraalbureau voor Schimmelcultures, Yeast Division, Delft.' Several yeast strains were kindly provided by Dr. R. Benham, Columbia University, New York. Nitrogen and carbon utilization was examined by the auxanographic method of Beijerinck (1889). An auxanographic procedure was also used to determine the vitamin requirements. Drops of solutions of vitamins were placed by means of a bacteriological loop on the surface of a solid medium containing KH2PO4, 0.1 per cent; MgSO4,0.05 per cent; glucose, 1 per cent; and (NH4)2SO4,0.1 per cent or its equivalent of norit-adsorbed acid hydrolyzate of casein. Circumscribed growth around the spot of deposition of the vitamin drop indicates a positive reponse to the vitamin. The diameter of the growth field is roughly proportional to the concentration of the vitamin in the drop (figure 1).2 In some instances nitrate assimilation was examined by growing the yeast on a medium composed as described above but with 0.1 per cent KNO3 as the sole source of nitrogen. After a growth period of 2 to 4 days the Griess-Ilosway reagent for nitrite was poured on the culture plate. Good growth on nitrate medium was always accompanied by a positive nitrite reaction.
منابع مشابه
Use of a urease test for the screening and identification of cryptococci.
The increase in mycological work by diagnostic laboratories has stimulated the development of simple and reliable procedures for routine screening and identification of fungus cultures. The writer has investigated the possibility of applying procedures for the diagnosis of yeasts other than the widely used sugar fermentation and assimilation tests. During earlier investigations it was found tha...
متن کاملAtypical forms of Cryptococcus neoformans in CSF of an AIDS patient.
The microscopic recognition of typical rounded capsulated yeasts in centrifuged cerebrospinal fluid (CSF), stained with India ink, is a common, rapid and effective method for the diagnosis of cryptococcal meningitis among AIDS patients. The presence of atypical forms of Cryptococcus neoformans var. neoformans in samples of CSF of an AIDS patient with cryptococcosis treated at the University Hos...
متن کاملMolecular Investigation of Diversity Normal Yeast Flora in Cow Raw Milk Industrial Farms of Tehran Province and Alborz
Background and Objective: Milk is a completely rich and complex environment suitable for different microorganisms including bacteria and Fungi. The majority of studies were conducted on milk-bacterial flora and aflatoxin-contaminations in dairy products; however, despite the importance of yeasts in usage as starter in the dairy industries and their effect on the taste of dairy products as well ...
متن کاملFoot bleb infection due to Rhodotorula mucilaginosa in a diabetic patient: Case report and review of the literature
Rhodotorula spp. are capsulated, pigmented, ubiquitous basidiomycetous yeasts causing different superficial and deep-seated opportunistic infections in the debilitated and immunocompromised patient. Earlier considered as saprophytes, fungi belonging to this genus are now considered as emerging pathogens. This is a report of foot bleb infection due to Rhodotorula mucilaginosa in a diabetic patient.
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of bacteriology
دوره 53 3 شماره
صفحات -
تاریخ انتشار 1947