Sun - Kee Kim 1 , Debby
نویسندگان
چکیده
The age-related differences in the synthesis of exportable and nonexportable proteins of the parotid salivary gland were compared in 2and 24-month-old rats. Parotid slices from these rats were incubated in the presence of [14C]leucine and the amount of radioactivity incorporated into the water-soluble proteins of the postmicrosomal supernatant was compared. The exportable and nonexportable proteins were identified by electrophoretic separation of these proteins by comparing the banding patterns of the gel preparations from unstimulated glands to those from the glands stimulated to secrete. The radioactivity determination in various protein bands from these rats indicated that the synthesis of exportable secretory proteins declined with age, while that of nonexportable proteins did not appear to change.
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