ECOLOGY AND BEHAVIOR New Restriction Fragment Length Polymorphisms in the Cytochrome Oxidase I Gene Facilitate Host Strain Identification of Fall Armyworm (Lepidoptera: Noctuidae) Populations in the Southeastern United States
نویسندگان
چکیده
Several restriction sites in the cytochrome oxidase I gene of fall armyworm, Spodoptera frugiperda (J.E. Smith), were identiÞed by sequence analysis as potentially being speciÞc to one of the two host strains. Strain speciÞcity was demonstrated for populations in Florida, Texas, Mississippi, Georgia, and North Carolina, with an AciI and SacI site speciÞc to the rice (Oryza spp.)-strain and a BsmI and HinfI site joining an already characterized MspI site as diagnostic of the corn (Zea mays L.)-strain. All four of these sites can be detected by digestion of a single 568-bp polymerase chain reaction-ampliÞed fragment, but the use of two enzymes in separate digests was found to provide accurateandrapiddeterminationof strain identity.Theeffectivenessof thismethodwasdemonstrated by the analysis of almost 200 adult and larval specimens from the Mississippi delta region. The results indicated that the corn-strain is likely to be the primary strain infesting cotton (Gossypium spp.) and that an unexpected outbreak of fall armyworm on the ornamental tree Paulownia tomentosa (Thunb.) Sieb. & Zucc. ex Steud. was due almost entirely to the rice-strain.
منابع مشابه
Ecology, Behavior and Bionomics First Genotyping of <i>Spodoptera frugiperda</i> (J. E. Smith) (Lepidoptera: Noctuidae) Progeny from Crosses between Bt-Resistant and Bt-Susceptible Populations, and 65-Locus Discrimination of Isofamilies
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