Development of a gene transfer system for the mycelia of Flammulina velutipes Fv-1 strain.
نویسندگان
چکیده
To develop a gene transformation method for Flammulina velutipes, we constructed a vector with hph gene under control of the trp1 gene promoter. The vector was integrated into protoplast derived from mycelia by the calcium-polyethylene glycol method, as it has not been reported for F. velutipes. Transformation efficiency was much improved when transformation was performed by the restriction enzyme mediated integration method.
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ورودعنوان ژورنال:
- Bioscience, biotechnology, and biochemistry
دوره 74 5 شماره
صفحات -
تاریخ انتشار 2010