An All-Glass Microfluidic Network with Integrated Amorphous Silicon Photosensors for on-Chip Monitoring of Enzymatic Biochemical Assay

نویسندگان

  • Francesca Costantini
  • Roald M Tiggelaar
  • Riccardo Salvio
  • Marco Nardecchia
  • Stefan Schlautmann
  • Cesare Manetti
  • Han J G E Gardeniers
  • Giampiero de Cesare
  • Domenico Caputo
  • Augusto Nascetti
چکیده

A lab-on-chip system, integrating an all-glass microfluidics and on-chip optical detection, was developed and tested. The microfluidic network is etched in a glass substrate, which is then sealed with a glass cover by direct bonding. Thin film amorphous silicon photosensors have been fabricated on the sealed microfluidic substrate preventing the contamination of the micro-channels. The microfluidic network is then made accessible by opening inlets and outlets just prior to the use, ensuring the sterility of the device. The entire fabrication process relies on conventional photolithographic microfabrication techniques and is suitable for low-cost mass production of the device. The lab-on-chip system has been tested by implementing a chemiluminescent biochemical reaction. The inner channel walls of the microfluidic network are chemically functionalized with a layer of polymer brushes and horseradish peroxidase is immobilized into the coated channel. The results demonstrate the successful on-chip detection of hydrogen peroxide down to 18 μM by using luminol and 4-iodophenol as enhancer agent.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Fabrication of integrated porous glass for microfluidic applications.

This paper presents a method for the fabrication of integrated porous silica layers in microfluidic channel networks by microfabrication techniques. Porous silica is obtained by anodization of silicon, followed by full conversion of the porous silicon network into porous silica by means of thermal oxidation. A series of experiments were performed with various channel layouts to determine the cr...

متن کامل

A highly sensitive a-Si photodetector array with integrated filter for optical detection in MEMS

This paper presents a highly sensitive photo-detector array deposited on a glass substrate with an integrated optical filter. The active element is a vertically integrated hydrogenated amorphous silicon photodiode featuring a dark current of less than 1e-10 A/cm for -3V bias voltage and a maximal quantum efficiency of 80% near 580 nm. The prototype was encapsulated and tested optically. It has ...

متن کامل

Fabrication of All Glass Bifurcation Microfluidic Chip for Blood Plasma Separation

An all-glass bifurcation microfluidic chip for blood plasma separation was fabricated by a cost-effective glass molding process using an amorphous carbon (AC) mold, which in turn was fabricated by the carbonization of a replicated furan precursor. To compensate for the shrinkage during AC mold fabrication, an enlarged photoresist pattern master was designed, and an AC mold with a dimensional er...

متن کامل

Miniaturizing DNA Sequencing Technology: Designing Microfluidic Channels for Performing Chemistry on Beads

page 152 Abstract: The availability of several complete organismal genomic sequences, made possible by drastic reductions in sequencing cost in concert with advances in sequencing technology, has revolutionized the nature of biological and biomedical research. Yet for this rapid progress to continue, and to enable the genomes of countless more organisms to be sequenced cheaply and efficiently, ...

متن کامل

Development and Application of Integrated Silicon-in-plastic Microfabrication in Polymer Microfluidic Systems

Title of Document: DEVELOPMENT AND APPLICATION OF INTEGRATED SILICON-IN-PLASTIC MICROFABRICATION IN POLYMER MICROFLUIDIC SYSTEMS Likun Zhu, Doctor of Philosophy, 2006 Directed By: Associate Professor Don L. DeVoe, Department of Mechanical Engineering Polymer-based microfluidic devices can offer a number of advantages over conventional devices, and have found many applications in chemical and bi...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره 7  شماره 

صفحات  -

تاریخ انتشار 2017