Cloning of parsley flavone synthase I.
نویسندگان
چکیده
A cDNA encoding flavone synthase I was amplified by RT-PCR from leaflets of Petroselinum crispum cv. Italian Giant seedlings and functionally expressed in yeast cells. The identity of the recombinant, 2-oxoglutarate-dependent enzyme was verified in assays converting (2S)-naringenin to apigenin.
منابع مشابه
Expression of parsley flavone synthase I establishes the flavone biosynthetic pathway in Arabidopsis thaliana.
Arabidopsis thaliana lacks the flavone biosynthetic pathway, probably because of a lack or low activity of a flavone synthase. To establish this biosynthetic pathway in Arabidopsis, we subjected this model plant to transformation with the parsley gene for flavone synthase type I (FNS-I). Transgenic seedlings expressing FNS-I were cultured in liquid medium with or without naringenin, and plant e...
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ورودعنوان ژورنال:
- Phytochemistry
دوره 58 1 شماره
صفحات -
تاریخ انتشار 2001