Calcium in sea urchin egg during fertilization.
نویسندگان
چکیده
Calcium plays a strikingly important role in two of the major events in developmental biology: cell activation and differentiation. In this review we begin with the location and quantity of intracellular calcium in sea urchin oocytes, and then discuss the changes that occur during fertilization and egg activation, placing special emphasis on the mobilization and redistribution of intracellular calcium. We also discuss the propagation of the calcium wave and the role of the burst of calcium on the process of reorganizing the egg cortex at fertilization.
منابع مشابه
Mechanism for Increase in Intracellular Concentration of Free Calcium in Fertilized Sea Urchin Egg
Intracellular free calcium concentration in the sea urchin egg was calculated to increase from 0.1 mM in an unfertilized egg to 1 mM in a fertilized egg 10 min after fertilization, based on measurement of the dissociation constant between free calcium and sea urchin egg homogenate. The dissociation constant between free calcium (dialyzable calcium) and homogenate of sea urchin eggs was measured...
متن کاملSource and sinks for the calcium released during fertilization of single sea urchin eggs
The source and sinks for the intracellular calcium released during fertilization were examined in single eggs from the sea urchin, Arbacia punctulata. Single eggs were microinjected with the calcium photoprotein, aequorin. The calcium-aequorin luminescence was measured with a microscope-photomultiplier or observed with a microscope-image intensifier-video system. In the normal egg a propagated ...
متن کاملIntracellular calcium release at fertilization in the sea urchin egg.
Fertilization or ionophore activation of Lytechinus pictus eggs can be monitored after injection with the Ca-sensitive photoprotein aequorin to estimate calcium release during activation. We estimate the peak calcium transient to reach concentrations of 2.5-4.5 & free calcium 45-60 set after activation and to last 2-3 min, assuming equal Ca2+ release throughout the cytoplasm. Calcium is release...
متن کاملActivation of sea urchin eggs by inositol phosphates is independent of external calcium.
We investigated the contribution of external calcium ions to inositol phosphate-induced exocytosis in sea urchin eggs. We show that: (a) inositol phosphates activate eggs of the sea urchin species Lytechinus pictus and Lytechinus variegatus independently of external calcium ions; (b) the magnitude and duration of the inositol phosphate induced calcium changes are independent of external calcium...
متن کاملThe Part Played By Inositol Trisphosphate and Calcium in the Propagation of the Fertilization Wave in Sea Urchin Eggs
Sea urchin egg activation at fertilization is progressive, beginning at the point of sperm entry and moving across the egg with a velocity of 5 ~tm/s. This activation wave (Kacser, H., 1955, J. Exp. Biol., 32:451-467) has been suggested to be the result of a progressive release of calcium from a store within the egg cytoplasm (Jaffe, L. E, 1983, Dev. Biol., 99:265-276). The progressive release ...
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ورودعنوان ژورنال:
- The International journal of developmental biology
دوره 34 1 شماره
صفحات -
تاریخ انتشار 1990