Induction of Testicular Sarcomas in Fischer Rats by Intratesticular Injection of Nickel Subsulfide1

Nickel subsulfide (Ni:S ) was injected in various amounts into the testis of adult Fischer rats for the study of the acute and chronic effects of Ni S on testicular cells. Rats given injections of 0.6 to 10 mg of Ni;S developed an immediate inflammatory response at the site of injection, followed by a delayed, slowly evolving coagulation necrosis of seminiferous tubules and intersti tial cells. The extent of testicular necrosis was dose dependent, but at doses of 5 or 10 mg of Ni ;S the rats invariably developed subtotal destruction of the testis. The testis became atrophie, without regeneration of sem iniferous tubules. No damage was seen in the other testis, and no systemic effects were noted. Malignant testicular neoplasms developed in 16 of 19 rats within 20 months after an injection of 10 mg of Ni;S:. These neo plasms were classified by light and electron microscopy as fibrosarcomas. malignant fibrous histiocytomas, and rhabdomyosarcomas. None of the testicular neoplasms was derived from germ cells or genital cord cells. The occurrence of rhabdomyosarcomas in the testis, an organ normally devoid of striated muscle, suggests that Ni :S, induces malignant transformation of undifferentiated, pluripotential mesenchymal cells.