Phytochemical, Anti-inflammatory and in vitro anticancer activities of Caesalpinia bonduc stem bark

Caesalpinia bonduc possess anti-inflammatory, anthelmintic, digestive, stomachic properties. The present study investigated anti-inflammatory and in vitro anticancer studies of stem bark of C.bonduc. The in vitro antiinflammatory study of different extracts were done by Protein denaturation method. The total ethanolic extract of stem bark of C.bonduc was investigated for in vivo anti-inflammatory activity (carrageenan induced rat paw oedema) at the doses 200 and 400mg/kg body weight in male Wister albino rats. The in vitro cytotoxicity study was done by Trypan blue dye exclusion technique in Daltons Ascites Lymphoma (DLA) cells at 200, 100, 50, 20, 10 μg/ml concentrations. Estimation studies by Folin Cio-calteau method and Aluminium chloride colorimetric method showed that phenolics and flavonoids are abundant in the stem bark. The in vitro and in vivo anti-inflammatory studies shows that TEE exhibits more anti-inflammatory effect which increases in a dose dependent manner. TEE exhibits 100% cytotoxicity even at 100 μg/ml concentrations. The present study revealed that presence high quantities of phenolics and flavonoids in the stem bark may be responsible for its anti-inflammatory anticancer properties.