Parameter Optimization of Enzyme Saccharification for low Lignin High Biomass Sorghum (CSV 15 X IS 21891)-1-1-1-1 and Estimation of Process Kinetics
نویسنده
چکیده
Optimization of Enzymatic saccharification conditions for acid treated bmr sorghum biomass was studied with the inhouse enzyme produced from Aspergillus sp.. Optimization of enzyme loading, substrate concentration, temperature and time yielded a maximum of 416.00 ± 0.55 mg/g sugars with an improved hydrolysis efficiency of 55.97 ± 0.45%. Protein adsorption onto lignocellulosic substance was investigated at pH 4.8 and at two different temperatures (4°C and 45°C) with the produced cellulase enzyme and compared with the commercial cellulase. The maximum adsorption capacities, the affinity constants and the binding strengths were more for sorghum biomass as compared to cellulose powder.
منابع مشابه
Sugarcane bagasse pretreatment using three imidazolium-based ionic liquids; mass balances and enzyme kinetics
UNLABELLED BACKGROUND Effective pretreatment is key to achieving high enzymatic saccharification efficiency in processing lignocellulosic biomass to fermentable sugars, biofuels and value-added products. Ionic liquids (ILs), still relatively new class of solvents, are attractive for biomass pretreatment because some demonstrate the rare ability to dissolve all components of lignocellulosic b...
متن کاملLignin triggers irreversible cellulase loss during pretreated lignocellulosic biomass saccharification
BACKGROUND Non-productive binding of enzymes to lignin is thought to impede the saccharification efficiency of pretreated lignocellulosic biomass to fermentable sugars. Due to a lack of suitable analytical techniques that track binding of individual enzymes within complex protein mixtures and the difficulty in distinguishing the contribution of productive (binding to specific glycans) versus no...
متن کاملCo-solvent pretreatment reduces costly enzyme requirements for high sugar and ethanol yields from lignocellulosic biomass.
We introduce a new pretreatment called co-solvent-enhanced lignocellulosic fractionation (CELF) to reduce enzyme costs dramatically for high sugar yields from hemicellulose and cellulose, which is essential for the low-cost conversion of biomass to fuels. CELF employs THF miscible with aqueous dilute acid to obtain up to 95 % theoretical yield of glucose, xylose, and arabinose from corn stover ...
متن کاملProduction of high concentrated cellulosic ethanol by acetone/water oxidized pretreated beech wood
BACKGROUND Lignocellulosic biomass is an abundant and inexpensive resource for biofuel production. Alongside its biotechnological conversion, pretreatment is essential to enable efficient enzymatic hydrolysis by making cellulose susceptible to cellulases. Wet oxidation of biomass, such as acetone/water oxidation, that employs hot acetone, water, and oxygen, has been found to be an attractive pr...
متن کاملNovel plants optimised for lignin, growth, and biofuel production via re-mutagenesis and co-expression analysis
The objectives of this project were to find novel genes that might be useful for optimising plant biomass to facilitate biofuel production. Since the phenolic polymer, lignin, surrounds and cross-links to other cell wall components, reducing the amount of lignin or altering its structure to make it easier to extract have become major targets for improving sugar release from plant biomass (sacch...
متن کامل