Tissue typing amniotic fluid cells: potential use for detection of contaminating maternal cells.

Human Amniotic Fluid Stem Cells: General Characteristics and Potential Therapeutic Applications

Introduction: Amniotic fluid contains a mixture of different cell types sloughed from the fetal skin, respiratory, alimentary and urogenital tracts, as well as the amnion membrane. As amniotic fluid develops prior to the process of gastrulation, many cells found in its heterogeneous population do not undergo lineage specialization. Therefore, amniotic fluid-derived mesenchymal stem cells (AF-MS...

Amniotic Fluid Stem Cells and Their Application in Cell-Based Tissue Regeneration

Amniotic fluid as a novel source of mesenchymal stem cells for therapeutic transplantation.

Human mesenchymal stem cells (MSCs) are multipotent stem cells, able to differentiate into multiple mesenchymal lineages.1-3 Previously, we have shown that human fetal lung–derived MSCs enhance the engraftment of human umbilical cord blood (UCB)– derived CD34 hematopoietic cells in nonobese diabetic–severe combined immunodeficiency mice.1 Here we show that secondtrimester amniotic fluid is an a...

The effect of human amniotic fluid on the survival and proliferation of human myeloma cell lines RPMI8226 and U266 in comparison with the fetal bovine serum

Background: Multiple Myeloma is a Plasma Cell Malignancy. Since the study of pathogenicity mechanisms and messenger pathways involved in the causative agent cells is important in the laboratory environment and close to the physiological environment of the body, therefore, the best environment for the study of cells in the laboratory environment, an environment most closely resembling the physio...

Potential Use of Amniotic Membrane Derived Scaffold for Cerebrospinal Fluid Applications

Scaffolds derived from decellularized tissues provide a natural microenvironment for cell culture. Embryonic cerebrospinal fluid (e-CSF) contains factors which play vital roles in development of the nervous system. This research was aimed to survey the effect of Wistar rat e-CSF on neural differentiation of bone marrow derived mesenchymal stem cells (BM-MSCs) cultured on the human amniotic memb...