Expression of phytase in yeast:
نویسندگان
چکیده
Background. Phytate (myo-inositol hexakisphosphate) is a naturally occurring compound in plants. Due to chelation of several dietary minerals, it acts as a potent anti-nutrient in foods. Degradation of phytate has previously been found to significantly improve absorption of nutritionally important minerals, such as iron and zinc, in the human intestine. By removing regulatory genes for phytase expression in Saccharomyces cerevisiae, high-phytase strains that constitutively degrade phytate have been constructed. A novel application is to use such strains for the degradation of phytate during gastrointestinal passage, and S. cerevisiae can constitute a suitable phytase carrier to the gastrointestinal tract since it has GRAS status (generally considered as safe).
منابع مشابه
Comparison of biochemical properties of recombinant phytase expression in the favorable methylotrophic platforms of Pichia pastoris and Hansenula polymorpha
Phytic acid is the dominant form of phosphorous in plant seeds. However, phytic acid cannot beutilized by animals and causes them serious phosphate deficiency. Utilization of phytase as ananimal feed additive can affect liberation of phosphor and its mineral availability. In this study,heterologous expression of the A. niger phyA gene was investigated in the methylotrophic yeastsP. pastoris and...
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