Characteristics of a Staphylococcal Phage Capable of Transduction.

Morse, M. L. (University of Colorado Medical Center, Denver) and J. W. LaBelle. Characteristics of a staphylococcal phage capable of transduction. J. Bacteriol. 83:775-780. 1962-The growth of staphylococcal phage 53, a temperate phage, has been studied. The latent periods in nutrient broth are: infected sensitive cells, 45 min; ultraviolet-induced lysogenic cells, 83 min. The burst size, as indicated by one-step growth studies and single-burst experiments, is 25 to 30 particles per lysing cell. Phage particles transducing streptomycin resistance and novobiocin resistance are produced at the same time as nontransducing particles. Some observations have been made on the host, Staphylococcus aureus NCTC 8511.