Development of single-nucleotide polymorphism markers for Bromus tectorum (Poaceae) from a partially sequenced transcriptome1
نویسندگان
چکیده
PREMISE OF THE STUDY Bromus tectorum (Poaceae) is an annual grass species that is invasive in many areas of the world but most especially in the U.S. Intermountain West. Single-nucleotide polymorphism (SNP) markers were developed for use in investigating the geospatial and ecological diversity of B. tectorum in the Intermountain West to better understand the mechanisms behind its successful invasion. METHODS AND RESULTS Normalized cDNA libraries from six diverse B. tectorum individuals were pooled and sequenced using 454 sequencing. Ninety-five SNP assays were developed for use on 96.96 arrays with the Fluidigm EP1 genotyping platform. Verification of the 95 SNPs by genotyping 251 individuals from 12 populations is reported, along with amplification data from four related Bromus species. CONCLUSIONS These SNP markers are polymorphic across populations of B. tectorum, are optimized for high-throughput applications, and may be applicable to other, related Bromus species.
منابع مشابه
Sequencing and de novo transcriptome assembly of Brachypodium sylvaticum (Poaceae)1
UNLABELLED PREMISE OF THE STUDY We report the de novo assembly and characterization of the transcriptomes of Brachypodium sylvaticum (slender false-brome) accessions from native populations of Spain and Greece, and an invasive population west of Corvallis, Oregon, USA. • METHODS AND RESULTS More than 350 million sequence reads from the mRNA libraries prepared from three B. sylvaticum genot...
متن کاملEvidence for resistance polymorphism in the Bromus tectorum – Ustilago bullata pathosystem: implications for biocontrol
Bromus tectorum L. (cheatgrass or downy brome) is an important exotic weed in natural ecosystems as well as in winter cereal cropland in semiarid western North America. The systemic, seedling-infecting head smut pathogen Ustilago bullata Berk. commonly infects cheatgrass stands, often at epidemic levels. We examined factors controlling U. bullata infection levels in greenhouse studies with pare...
متن کاملNative Perennial Grasses Show Evolutionary Response to Bromus tectorum (Cheatgrass) Invasion
Invasive species can change selective pressures on native plants by altering biotic and abiotic conditions in invaded habitats. Although invasions can lead to native species extirpation, they may also induce rapid evolutionary changes in remnant native plants. We investigated whether adult plants of five native perennial grasses exhibited trait shifts consistent with evolution in response to in...
متن کاملUsing High-Resolution Future Climate Scenarios to Forecast Bromus tectorum Invasion in Rocky Mountain National Park
National Parks are hallmarks of ecosystem preservation in the United States. The introduction of alien invasive plant species threatens protection of these areas. Bromus tectorum L. (commonly called downy brome or cheatgrass), which is found in Rocky Mountain National Park (hereafter, the Park), Colorado, USA, has been implicated in early spring competition with native grasses, decreased soil n...
متن کاملApplication of single-nucleotide polymorphism (SNP) as a molecular marker in the study of genetic diversity of aquatic populations
Genetic diversity is one of the important and essential characteristics of any population for its survival. The study of genetic variation in different populations of aquatic organisms is of particular importance in order to protect, stabilize and manage their stocks. Based on studies conducted in recent years, molecular markers have proven that they can be used as indicators of the genetic div...
متن کامل