Target cell-restricted and -enhanced apoptosis induction by a scFv:sTRAIL fusion protein with specificity for the pancarcinoma-associated antigen EGP2.

Target cell-restricted and -enhanced apoptosis induction by an scFv:sTRAIL fusion protein with specificity for the pancarcinoma- associated antigen EGP2

The apparent tumour selective apoptosis inducing activity of recombinant soluble TRAIL has aroused much interest for use in clinical application. However, to fully exploit its therapeutic potential the characteristics of both the TRAIL receptor system and sTRAIL should be taken into account. Firstly, the wide spread expression of the various TRAIL receptors throughout the human body; secondly, ...

Exceptionally potent anti-tumor bystander activity of an scFv:sTRAIL fusion protein with specificity for EGP2 toward target antigen-negative tumor cells.

Previously, we reported on the target cell-restricted fratricide apoptotic activity of scFvC54:sTRAIL, a fusion protein comprising human-soluble tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) genetically linked to the antibody fragment scFvC54 specific for the cell surface target antigen EGP2. In the present study, we report that the selective binding of scFvC54:sTRAIL to EGP2-...

Simultaneous inhibition of EGFR signalling and enhanced activation of TRAIL-R-mediated apoptosis induction by an scFv:sTRAIL fusion protein with specificity for human EGFR

Construction and characterization of a bispecific diabody for retargeting T cells to human carcinomas.

We describe the construction of a recombinant bispecific antibody fragment in the diabody format with specificity for both the well-established human pancarcinoma associated target antigen EGP2 (epithelial glycoprotein 2, also known as the CO17-1A antigen or KSA) and the CD3epsilon chain of human TCR/CD3 complex. The murine anti-EGP2 (MOC31) single chain variable fragment (scFv) and the humaniz...

Immunogencity of HSA-L7/L12 (Brucella abortus Ribosomal Protein) in an Animal Model

Background: The immunogenic Brucella abortus ribosomal protein L7/L12 is a promising candidate antigen for the development of subunit vaccines against brucellosis. Objective: This study was aimed to evaluate the protection of recombinant Human Serum Albumin (HAS)-L7/L12 fusion protein in Balb/c mice. Methods: The amplified L7/L12 gene was cloned in pYHSA5 vector, pYHSA5-L7/L12 construct was tra...