Transcriptome of barley under three different heavy metal stress reaction
نویسندگان
چکیده
In the present study, we used Illumina sequencing technology (HiSeq 2000) to sequence the transcriptome of barley (Hordeum vulgare L., cv. Morex) under three different heavy metal stress conditions: copper, zinc and cadmium. For each of those metals, the concentration causing a 50% inhibitory effect for root growth (EC50) was determined. We sequenced the total RNA of both roots and shoots from barley with and without heavy metal treatments in three replicates. Raw reads of the transcriptome project have been deposited in NCBI's BioProject accession number PRJNA382490. The obtained transcriptomic data will be useful for further studies focusing on heavy metal tolerance and comparative transcriptome analysis in barley.
منابع مشابه
Identification of Glutathione S-Transferase (GST) Genes from a Dark Septate Endophytic Fungus (Exophiala pisciphila) and Their Expression Patterns under Varied Metals Stress
Glutathione S-transferases (GSTs) compose a family of multifunctional enzymes that play important roles in the detoxification of xenobiotics and the oxidative stress response. In the present study, twenty four GST genes from the transcriptome of a metal-tolerant dark septate endophyte (DSE), Exophiala pisciphila, were identified based on sequence homology, and their responses to various heavy m...
متن کاملEvaluation of Biochemical Traits and Dry Matter Remobilization of Barley (Hordeum vulgare L.) in Relay Intercropping with Chickpea (Cicer arietinum L.) under Deficit Water Stress Conditions
In order to investigate the effect of deficit water stress and different combinations of relay intercropping of chickpea with barley on some biochemical traits and grain yield, a field experiment was conducted at College of Agriculture and Natural Resources of Darab, Shiraz University during 2017-2018 cropping season, as split plot based on a randomized complete block design with three replicat...
متن کاملTranscriptome Profiling of Louisiana iris Root and Identification of Genes Involved in Lead-Stress Response
Louisiana iris is tolerant to and accumulates the heavy metal lead (Pb). However, there is limited knowledge of the molecular mechanisms behind this feature. We describe the transcriptome of Louisiana iris using Illumina sequencing technology. The root transcriptome of Louisiana iris under control and Pb-stress conditions was sequenced. Overall, 525,498 transcripts representing 313,958 unigenes...
متن کاملThe effects of heavy metal mercury on some physiological responsses in Nicotiana tabacum
One of the major abiotic stresses that negatively affects plants is the presence of heavy metals. Soil pollution with heavy metals, resulting from the industrial development and use of fertilizers containing heavy metals, has become a major environmental concern in human societies. Mercury is a toxic heavy metal that causes pollution in agricultural lands. Accumulation of Hg by plants may disru...
متن کاملHeavy metal induced physiological changes in the antioxidative response system
Heavy metal stress induced alterations in the activities of several representatives of the enzymatic antioxidant defense system such as guiacol peroxidase (POD), catalase (CAT) and ascorbate peroxidase (APX) were comparatively studied in barley seedlings treated by Zn and Cd. Although roots were the main sites of metal accumulation, the oxidative damage was detrimental mainly in the leaves. Our...
متن کامل