De Novo Genome Assembly of Grapevine Yellow Speckle Viroid 1 from a Grapevine Transcriptome
نویسندگان
چکیده
Grapevine yellow speckle viroid 1 (GYSVd1), which is a member of the genus Apscaviroid, causes yellow speckle disease in grapevines. Here, we report the complete de novo genome assembly of GYSVd1 from the grapevine transcriptome and identified 10 single nucleotide polymorphisms of GYSVd1 across the grapevine populations.
منابع مشابه
Complete Genome Sequences of Grapevine Yellow Speckle Viroid 1 and Hop Stunt Viroid Assembled from the Transcriptome of Ixeridium dentatum Plants
Here, we report complete genome sequences of grapevine yellow speckle viroid 1 (GYSVd1) and hop stunt viroid (HSVd), members of the family Pospiviroidae, assembled from the transcriptome data generated from Ixeridium dentatum plants. To our knowledge, this is the first report of GYSVd1 and HSVd in I. dentatum.
متن کاملتعیین مشخصات مولکولی و تبارزایی ویروئید لکه زردی-1 مو (Grapevine yellow speckle viroid-1) از تاکستان¬های شمال¬غرب کشور
بهمنظور تعیین مشخصات مولکولی و بررسی ساختارهای ثانویه ویروئید لکه زردی- 1 مو، 137 نمونه از تاکستانهای استانهای اردبیل، آذربایجان شرقی و آذربایجان غربی جمعآوری گردید. اسید نوکلئیک کل از بافت برگ نمونهها استخراج و پس از ساخت دیانای مکمل با استفاده از آغازگر ششنوکلئوتیدی تصادفی، طول کامل این ویروئید با روش پیسیآر و با استفاده از آغازگرهای اختصاصی ویروئید لکه زردی -1 مو، تکثیر شد. مح...
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