The Isolation and Assay of Hypertensin from Blood
نویسندگان
چکیده
A method has been described for isolation and assay of hypertensin from the blood of dogs. An ethanol filtrate of blood drawn from the femoral artery is prepared and concentrated by evaporation. The hypertensin is extracted into n-butanol from which it is adsorbed into alumina, and subsequently eluted with dilute ethanol. The eluate is then evaporated to dryness, dissolved in water, and assayed by intravenous injection into rats.
منابع مشابه
Isolation and Assay of Hypertensin from Blood
The identification of the vasopressor substance responsible for the elevation of the blood pressure in renal hypertension would be a contribution to the knowledge of the mechanism causing this phenomenon. One of the pressor substances suspected of being the chemical mediator of hypertension is the polypepfide hypertensin which is produced by the enzymatic action of renin upon its substrate, a-2...
متن کاملThe isolation of hypertensin from the circulating blood of dogs by dialysis in an artificial kidney.
T HE PRESENT VIEW of the mode of development of experimental renal hypertension' is that a chemical mechanism causes the elevation of the blood pressure. The components of this mechanism are the renal enzyme, renin, its plasma substrate, hypertensinogen, and the vasopressor substance, hypertensin, resulting from the interaction of renin and hypertensinogen. It is of paramount importance for the...
متن کاملAssay of renin and hypertension with the isolated guineapig ileum.
THE PROBLEM of the participation of the renin-hypertensinogen-hypertensin system in the mechanism of desoxycorticosterone-hypei'tension might be attacked successfully only by the application of a sensitive method of renin assay, for it was clear from a previous study that if renin is liberated from the dexosycorticosterone-hypertensive kidney, the amount is small. Unfortunately the assay method...
متن کاملThe Assay of Hypertensin from the Arterial Blood of Normotensive and Hypertensive Human Beings
Hypertensin has been assayed in the blood of patients with normal blood pressure and in those with essential hypertension in both the benign and malignant phases. 250 ml. samples of arterial blood were obtained, chemically purified, and concentrated to a volume of 1 ml. These extracts were then assayed in anesthetized rats. The concentrations of hypertensin in the blood of patients with the mal...
متن کاملThe isolation of hypertensin from the circulating blood of normal dogs with experimental renal hypertension by dialysis in an artificial kidney.
T HE PRESENT paper is a report on experimental procedures designed to determine whether hypertensin is the vasoconstrictor substance responsible for the elevation of blood pressure in. dogs with benign experimental renal hypertension. Previous work' has shown that a pressor material similar in all general respects to hypertensin can be dialyzed out of the circulating blood of dogs by the use of...
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ورودعنوان ژورنال:
- The Journal of Experimental Medicine
دوره 95 شماره
صفحات -
تاریخ انتشار 1952