Controversy: Preimplantation Genetic Diagnosis
نویسنده
چکیده
bjective: To review a 12-year experience of the world’s three largest preimplantation genetic diagnosis PGD) centers. esign: Multicenter analysis of the clinical outcome of PGD. etting: In vitro fertilization programs at the Reproductive Genetics Institute, Chicago, Illinois; Saint arnabas Medical Center, West Orange, New Jersey; and SISMER, Bologna, Italy. atient(s): Poor-prognosis IVF patients, patients carrying balanced chromosomal translocations, and couples t risk for producing children with Mendelian disorders. ntervention(s): In vitro fertilization, intracytoplasmic sperm injection, polar body removal, blastomere iopsy, and ET. ain Outcome Measure(s): DNA or chromosomal analysis of biopsied polar bodies or blastomeres, mplantation and clinical pregnancy rates, and live-born pregnancy outcome. esult(s): A total of 754 babies have been born as a result of 4,748 PGD attempts, which shows the expanded pplication and the practical relevance of PGD for single-gene disorders, chromosomal aneuploidies and ranslocations, late-onset diseases with genetic predisposition, and nondisease testing in couples at need for uman leukocyte antigens-matched offspring for treatment of affected siblings. onclusion(s): Preimplantation genetic diagnosis is evolving to become a clinical option for couples at risk or producing offspring with Mendelian diseases, has a positive numerical impact in standard assisted eproduction practices through aneuploidy testing, and reduces by at least fourfold the spontaneous abortion ate in couples carrying translocations. (Fertil Steril 2004;82:292–4. ©2004 by American Society for eproductive Medicine.) ey Words: Preimplantation genetic diagnosis, aneuploidy testing, IVF efficiency, chromosomal transloca-
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