Compact Quantitative Proteomics Workflow Combining SILAC Labeling, Chromatographic pre-fractionation and CESI-MS with Neutral Capillary Surface
نویسندگان
چکیده
• Fully automated reverse-phase chromatography fractionation and CESI-MS analyses • Increased coverage of modified peptides without sample enrichment • Reduced workload due to the single in-solution digestion • Ultralow sample consumption (c.a. 40nL) per analysis Klaus Faserl,1 Leopold Kremser,1 Martin Müller,2 David Teis,2 László Hajba,3 András Guttman4 and Herbert H. Lindner1 1Division of Clinical Biochemistry, Biocenter, Innsbruck Medical University, Innrain 80-82, A-6020 Innsbruck, Austria 2Division of Cell Biology, Biocenter, Innsbruck Medical University, Innrain 80-82, A-6020 Innsbruck, Austria 3MTA-PE Translational Glycomics Research Group, University of Pannonia, Veszprem, Hungary 4Sciex, Brea, CA 92822
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