7, A Candidate Tumor Suppressor on Chromosome 14, Regulates Pathways of DNA

ownload onic lymphocytic leukemia deletion gene 7 (Clld7) is a candidate tumor suppressor on chromosome . Clld7 encodes an evolutionarily conserved protein that contains an RCC1 domain plus broad complex, ack, bric-a-brac (BTB), and POZ domains. In this study, we investigated the biological functions of Clld7 n in inducible osteosarcoma cell lines. Clld7 induction inhibited cell growth, decreased cell viability, and sed γ-H2AX staining under conditions of caspase inhibition, indicating activation of the DNA damage/ pathway. Real-time PCR analysis in tumor cells and normal human epithelial cells revealed Clld7 target that regulate DNA repair responses. Furthermore, depletion of Clld7 in normal human epithelial cells genes conferred resistance to apoptosis triggered by DNA damage. Taken together, the biological actions of Clld7 are consistent with those of a tumor suppressor. Cancer Res; 70(22); 9434–43. ©2010 AACR.