Identification of virus-specific oligoclonal bands in subacute sclerosing panencephalitis by immunofixation after isoelectric focusing and peroxidase staining.

Oligoclonal IgG antibody response in the central nervous system to different measles virus antigens in subacute sclerosing panencephalitis.

Agarose electrophoresis of cerebrospinal fluid from two patients with subacute sclerosing panencephalitis revealed in both cases several homogeneous bands in the intermediate and basic gammaglobulin region. The bands were identified as IgG with various degrees of kappa or lambda light-chain restriction in crossed immunoelectrophoresis. Homogeneous IgG bands were separated by preparative agarose...

Detection of IgG oligoclonal bands in unconcentrated CSF by isoelectric focusing in ultrathin polyacrylamide gel, direct antiserum immunofixation and silver nitrate staining.

Isoelectric focusing of proteins in ultrathin polyacrylamide gel (0.4 mm), followed by direct immunofixation with monospecific antisera and silver nitrate staining, is a highly specific, sensitive and simple method for the detection of oligoclonal IgG in unconcentrated CSF samples. The ultrathin polyacrylamide gels have several advantages, i.e. significantly smaller amounts of reagents are requ...

Isolation and characterization of a defective measles virus from brain biopsies of three patients in Iran with subacute sclerosing panencephalitis

Cloning the antibody response in humans with chronic inflammatory disease: immunopanning of subacute sclerosing panencephalitis (SSPE) brain sections with antibody phage libraries prepared from SSPE brain enriches for antibody recognizing measles virus antigens in situ.

In central nervous system (CNS) infectious and inflammatory diseases of known cause, oligoclonal bands represent antibody directed against the causative agent. To determine whether disease-relevant antibodies can be cloned from diseased brain, we prepared an antibody phage display library from the brain of a human with subacute sclerosing panencephalitis (SSPE), a chronic encephalitis caused by...

LETTERS TO THE EDITOR In vivo cerebral proton MRS in a case of subacute sclerosing panencephalitis Subacute sclerosing panencephalitis

Subacute sclerosing panencephalitis (SSPE) is a rare encephalopathy caused by persistent defective measles virus in the CNS. Brain lesions may involve all regions of the CNS. The pathophysiological events associated with the disease are characterised by a perivascular infiltration by monocytes and astrocytic proliferation, neuronal degeneration, and demyelination. The exploration of SSPE by bra...