Morphological , biochemical and physiological studies on the preservation of buffalo ( Bubalus bubalis ) spermatozoa in different modified dilutors : Effects of chloroquine - diphosphate

A morphological, biochemical and physiological study has been made of the effects of chloroquine-diphosphate (a membrane stabilizer) on the preservation of buffalo spermatozoa in five modified dilutors designated here as Al’ A2’ A3, A4 and CAW. In almost all the five dilutors, there was significant (p< 0.01) progressive increase in the percentage of sperm abnormalities and the release of enzymes (hyaluronidase, glutamateoxaloacetic transaminase, alcohol, malate, lactate and sorbitol dehydrogenases) from spermatozoa into the media, and progressive decrease in the percentage of sperm motility and of the live sperms, with the increasing time of storage. Supplementation of these dilutors with 105 M chloroquine-diphosphate significantly (p ! 0.01) lowered the percentage of sperm abnormalities and the release of various enzymes, and increased the percentages of sperm motility and of live sperm in most of the cases. Drug capacity varied in different dilutors. It was maximum in A, (egg yolk citrate, EYC) and minimum in citric acid whey (CAW).