Effect of Condensed Tannin in Lotus Corniculatus and Lotus Pedunculatus on Digestion of Rubisco in the Rumen

The in vitro precipitation of ribulose-1,5-bisphosphate carboxylase (Rubisco) by condensed tannin (CT) extracted from Lotus corniculatus and Lotus pedunculatus and the effect of these CT on the in vitro rumen degradation of Rubisco was used to compare the reactivity of these CT. The chemical structure of CT from Lotus corniculatus was homogenous with epicatechin stereochemistry and mostly procyanidin units. The CT from Lotus pedunculatus was heterogenous with mostly prodelphinidin units. The amount of CT required to precipitate all the Rubisco when total soluble leaf protein was incubated with CT from Lotus corniculatus and Lotus pedunculatus was similar. Although CT from both species were able to reduce the in vitro degradation of Rubisco, CT from Lotus corniculatus was less effective than CT from Lotus pedunculatus at reducing this degradation.