Effect of gaseous conditions on isolation and growth of Ureaplasma urealyticum on agar.

نویسنده

  • J A Robertson
چکیده

Five laboratory-adapted strains of Ureaplasma urealyticum showed comparable colony counts when incubated in eight gaseous environments including air, in different concentrations of carbon dioxide, and under anaerobiosis. For primary isolation on genital mycoplasma agar 95% N2-5% CO2 gave 100% correlation with growth in bromothymol blue broth, whereas certain strains failed to grow on agar incubated in air, the TABCO2 system (Lab-Tek Division, Miles Laboratories, Ltd., Rexdale, Canada), 100% CO2, or under anaerobiosis.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Evaluation of PPLO, A7B, E, and NYC agar media for the isolation of Ureaplasma urealyticum and Mycoplasma species from the genital tract.

Four agar media (PPLO, NYC, A7B, and E) which are commonly used for the isolation of urogenital tract Mycoplasma species and Ureaplasma urealyticum were compared by culturing swabs of the endocervix of 334 pregnant women on all four media. To permit growth of both Mycoplasma and U. urealyticum, selective ingredients were omitted from the media tested. A7B and E agar were both satisfactory for t...

متن کامل

Detection of Ureaplasma Urealyticum in Clinical Samples from Infertile Women by Polymerase Chain Reaction

Genital Ureaplasma urealyticum infection is considered to be a sexually transmitted infection. The bacterium has been found to be involved in PID, chorioamnionitis, urethritis, respiratory distress syndrome and pneumonia in newborn, abortion and infertility. U. urealyticum infections not only jeopardize fertility but also pose a risk for infertility treatment and resulting pregnancies. The purp...

متن کامل

Evaluation of Mycotrim-GU for isolation of Mycoplasma species and Ureaplasma urealyticum.

The Mycotrim-GU (Hana Biologics, Berkeley, Calif.) biphasic culture system and a conventional system were compared for their ability to detect Ureaplasma urealyticum and Mycoplasma species in 100 clinical specimens. Both systems detected 18 Mycoplasma spp. isolates. The average colony detection time was 1.9 days with the Mycotrim-GU and 2.3 days with the conventional system. The Mycotrim-GU aga...

متن کامل

An evaluation of the usefulness of a culture system for the detection of Ureaplasma urealyticum in the endotracheal aspirates of neonates.

In this study, we evaluated the usefulness of a culture system used in our laboratory for the detection of Ureaplasma urealyticum in the endotracheal aspirates of neonates. Ureaplasmal broth was used to enhance the growth of U. urealyticum followed by observation of colonies on A7 agar. Of the 68 samples of endotracheal aspirates tested, 60 gave positive indication of urease activity by the bro...

متن کامل

Unusual colonies of Ureaplasma urealyticum (T mycoplasmas) in primary agar cultures of certain urine specimens.

The existence of unusual colonies of Ureaplasma urealyticum (T mycoplasmas) in primary agar cultures of certain urine specimens is reported and their morphology is illustrated.

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Journal of clinical microbiology

دوره 15 2  شماره 

صفحات  -

تاریخ انتشار 1982