Protein chain initiation in wheat embryo. Resolution and function of the soluble factors.
نویسندگان
چکیده
The complete resolution of the initiation and transfer factors of wheat embryo supernatant is described. Two factors function in chain initiation, as ascertained by (a) their requirement for the formation of a ribosome-messenger RNA complex whose capacity for ammo acid incorporation is insensitive to aurintricarboxylic acid; and (b) their participation in the tobacco mosaic virus RNA-dependent binding of methionyl transfer RNA to ribosomes. The remaining factors, Tl(E) and TZ(E), catalyze the poly(U)-dependent polymerization of phenylalanine, establishing their function primarily in chain elongation. All four factors are required for tobacco mosaic virus RNA-dependent amino acid incorporation.
منابع مشابه
Protein chain initiation in wheat embryo.
The complete resolution of the initiation and transfer factors of wheat embryo supernatant is described. Two factors function in chain initiation, as ascertained by (a) their requirement for the formation of a ribosome-messenger RNA complex whose capacity for ammo acid incorporation is insensitive to aurintricarboxylic acid; and (b) their participation in the tobacco mosaic virus RNA-dependent ...
متن کاملProtein chain initiation by methionyl-tRNA in wheat embryo.
The mechanism of protein chain initiation has been investigated in a cell-free amino acid incorporation system from wheat embryos dependent on tobacco mosaic virus RNA. Analysis of the N-termini of the labeled peptide products of short-term incubations showed the presence of unblocked methionine. In addition, methionyl-tRNA (Met-tRNA) could be bound to ribosomes at 1.3 mM Mg(++) in a reaction r...
متن کاملتأثیر تنش کم آبی، ازدیاد دیاکسید کربن و اشعه ماورای بنفش بر صفات کیفی برگ پرچم گندم دوروم ( Triticum turgidum L. var. durum Desf.)
Water deficit, ultraviolet radiation and CO2 concentration enhancement are three environmental stresses that affect nutrition of human in future. This research was conducted in the Research Greenhouse of Faculty of Agriculture, Tarbiat Modares University in 2006, in order to study leaf qualitative traits of durum wheat under different levels of carbon dioxide (400 and 900 ppm), ultraviolet radi...
متن کاملتأثیر تنش کم آبی، ازدیاد دیاکسید کربن و اشعه ماورای بنفش بر صفات کیفی برگ پرچم گندم دوروم ( Triticum turgidum L. var. durum Desf.)
Water deficit, ultraviolet radiation and CO2 concentration enhancement are three environmental stresses that affect nutrition of human in future. This research was conducted in the Research Greenhouse of Faculty of Agriculture, Tarbiat Modares University in 2006, in order to study leaf qualitative traits of durum wheat under different levels of carbon dioxide (400 and 900 ppm), ultraviolet radi...
متن کاملEfficient synthesis of rabbit globin in a cell-free system from wheat embryo.
A cell-free preparation isolated from wheat embryos has been used successfully for the analysis of protein chain initiation with natural mRNA’s primarily of plant viral origin [l-4]. A tacit assumption in these studies has been that the ribosome-mRNA attachment reaction with the viral RNA’s was equivalent to the reaction with cellular mRNA’s. In the present communication we test this assumption...
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ورودعنوان ژورنال:
- The Journal of biological chemistry
دوره 247 8 شماره
صفحات -
تاریخ انتشار 1972