Integration of a Gradient Elution System for Pressure-driven Liquid Chromatography with Mems Fabricated Efficient Pillar Array Columns

This paper reports a gradient elution system for pressure-driven liquid chromatography (LC) on a chip. We have already reported the microfabrication of efficient pillar array columns with low dispersion turns. For faster and more efficient analysis, we fabricated a gradient elution system with a cross-Tesla mixer for the effective mixing of two solutions on a chip with a separation channel of pillar array columns and a sample injection channel. The results of the separation of two dyes under gradient elution conditions actually showed that the developed chip performed better, faster and more efficiently.