Lethal hydroxyl radical accumulation by a lactococcal bacteriocin, lacticin Q.
نویسندگان
چکیده
The antimicrobial mechanism of a lactococcal bacteriocin, lacticin Q, can be described by the toroidal pore model without any receptor. However, lacticin Q showed different degrees of activity (selective antimicrobial activity) against Gram-positive bacteria even among related species. The ability of lacticin Q to induce pore formation in liposomes composed of lipids from different indicator strains indicated that its selective antimicrobial activity could not be attributed only to membrane lipid composition. We investigated the accumulation of deleterious hydroxyl radicals after exposure to lacticin Q as a contributing factor to cell death in the indicator strains. When lacticin Q of the same concentration as the MIC or minimum bactericidal concentration was added to the indicator cultures, high levels of hydroxyl radical accumulation were detected. Treatment with hydroxyl radical scavengers, thiourea and 2,2'-bipyridyl, decreased the levels of hydroxyl radical accumulation and recovered cell viability. These results suggest that, with or without pore formation, the final antimicrobial mechanism of lacticin Q is the accumulation of hydroxyl radicals, which varies by strain, resulting in the selective antimicrobial activity of lacticin Q.
منابع مشابه
Title; Lethal Hydroxyl Radical Accumulation by a Lactococcal Bacteriocin, Lacticin Q Running Title; a New Antimicrobial Mechanism of Lacticin Q 3 4 Introduction
23 The antimicrobial mechanism of a lactococcal bacteriocin, lacticin Q, can be 24 described by the toroidal pore model without any receptor. However, lacticin Q 25 showed different degrees of activity (selective antimicrobial activity) against 26 gram-positive bacteria even among related species. The ability of lacticin Q to induce 27 pore formation in liposomes composed of lipids from differe...
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ورودعنوان ژورنال:
- Antimicrobial agents and chemotherapy
دوره 57 8 شماره
صفحات -
تاریخ انتشار 2013