Rapid in Vitro Plant Regeneration from Nodal Explants of Withania somnifera (L.) Dunal: a Valuable Medicinal Plant
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چکیده
Withania somnifera (L.) Dunal is high demanding valuable medicinal plants which have pharmaceuticals properties, known for its with anolides. Nodal segment explants of W. somnifera when cultured on MS (Murashige and skoog’s, 1969) medium horizontally containing different concentration(1-3mg/l) of 6-benzylaminopurine (BAP), kinetin (Kn) and thidiazuron (TDZ) produced multiple shoots on both axillary ends of explants when kept horizontally in the medium, within 3 weeks of culture incubation. Maximum multiple shoots was found in MS medium supplemented with (2.0mg/l) BAP as it induced an average of 21 ± 0.0.57 shoots per explants and the maximum shoot length (5.24 ± 0.20) cm. Successful in vitro rooting was induced from cut end of the microshoots when placed on halfstrength MS medium supplemented with (5.0mg/l) Indole-3-butyric acid (IBA). The regenerated shoots with well developed root system were successfully acclimatized and established in pots containing soil:leaf manure (3:1) and grown under greenhouse conditions with
منابع مشابه
High-Frequency in Vitro Direct Shoot Regeneration from Nodal Explants of Hyssop Plant (Hyssopus officinalis L.)
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