Title: Novel DNA-vaccine for PRRSV with improved heterologous protection. NPB project: #11-089 Investigator:

Title: Induction Of Cross-Protective Immunity Without Exposure To Live PRRSV - NPB #08-197 Investigator:

A Synthetic Porcine Reproductive and Respiratory Syndrome Virus Strain Confers Unprecedented Levels of Heterologous Protection.

UNLABELLED Current vaccines do not provide sufficient levels of protection against divergent porcine reproductive and respiratory syndrome virus (PRRSV) strains circulating in the field, mainly due to the substantial variation of the viral genome. We describe here a novel approach to generate a PRRSV vaccine candidate that could confer unprecedented levels of heterologous protection against div...

Chimeric porcine reproductive and respiratory syndrome virus containing shuffled multiple envelope genes confers cross-protection in pigs.

The extensive genetic diversity of porcine reproductive and respiratory syndrome virus (PRRSV) strains is a major obstacle for vaccine development. We previously demonstrated that chimeric PRRSVs in which a single envelope gene (ORF3, ORF4, ORF5 or ORF6) was shuffled via DNA shuffling had an improved heterologous cross-neutralizing ability. In this study, we incorporate all of the individually-...

Title : The influence of maternal antibody against porcine reproductive and respiratory syndrome virus ( PRRSV ) infection and the effect of a killed PRRSV vaccine in increasing the level of maternal antibody – NPB

Killed porcine reproductive and respiratory syndrome virus (PRRSV) vaccine (KV) was administered to sows pre-farrowing to determine if this protocol would increase the magnitude and duration of maternally derived antibody (MDA) and protective immunity of piglets. Sows from a PRRSV positive herd with no history of KV use were vaccinated at 60 and 75 days of gestation. Serological responses were ...

Broadening the Heterologous Cross-Neutralizing Antibody Inducing Ability of Porcine Reproductive and Respiratory Syndrome Virus by Breeding the GP4 or M genes

Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most economically important swine pathogens, which causes reproductive failure in sows and respiratory disease in piglets. A major hurdle to control PRRSV is the ineffectiveness of the current vaccines to confer protection against heterologous strains. Since both GP4 and M genes of PRRSV induce neutralizing antibodies, in...