Microfluidic Assay to Compare Secretion Vs Contact-based Cell-cell Interactions Using Dynamic Isolation Control
نویسندگان
چکیده
We report cell-cell interaction studies utilizing a microfluidic chip capable of capturing cell pairs of different types into culture microwells and isolating the captured cells for a given amount of time using pneumatic valves. We have quantified the capture efficiency and confirmed cell viability and interaction in the microwells. We successfully distinguished interaction modes in the fabricated devices: secretion-based and contact-based. C2C12 cells, when cocultured with PC3, showed increased proliferation due to PC3 secretions. Also, it was indentified that C2C12 proliferation is increased through contact with endothelial cells by comparing proliferation in both secretion only and contact only systems, respectively.
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