Correlative Fluorescence- and Scanning, Transmission Electron Microscopy for Biomolecular Investigation

These days, cellular and molecular biology are closely integrated due to the development of novel correlative biomolecular microscopy platforms, even though this avant-garde vision of combining different imaging tools has been around since the late 1960s. The current boom and successful application of correlative imaging methods to dissect the cell’s internal structure is progressing faster than ever as witnessed by the growth in the number of papers dealing with correlative microscopy. In this chapter we outline in-depth two straightforward methods to combine fluorescent and electron microscopic information of a single cell by using confocal laser-, scanning electronand transmission electron microscopy, and discuss how these methods contribute to our understanding of biomolecular cell processes, such as cell movement and cytoskeleton dynamics.