Variation to radiation resistance in Nocardia corallina.

Slime as a possible factor in cell clumping in Nocardia corallina.

Work on the nuclear cytology and life cycle of Nocardia corallina has previously been reported (Webb et al., 1954; Webb and Clark, 1957; Clark and Frady, 19.57). Extensive studies on the radiation response, physiology, and genetics of this organism have also been carried ouit in this laboratory. Throughout this work, one of the main problems involved in the experimental approach has been the el...

Metabolic pathway for poly(3-hydroxybutyrate-co-3-hydroxyvalerate) formation in Nocardia corallina: inactivation of mutB by chromosomal integration of a kanamycin resistance gene.

The gene encoding the large subunit of the methylmalonyl-coenzyme A (CoA) mutase in Nocardia corallina (mutBNc) was cloned. A 4.3-kbp BamHI fragment containing almost the entire mutBNc was identified by Southern hybridization experiments employing a digoxigenin-labeled probe deduced from mutB of Streptomyces cinnamonensis, mutBNc was interrupted by insertion of a kanamycin resistance gene block...

Studies on the nutrition of Nocardia corallina.

The nutritional requirements of Streptothrix corallinus (Nocardia corallina, Breed et al., 1948) were studied by Reader (1928) who showed that growth in a medium containing glucose, ammonium sulfate and inorganic salts was stimulated by the addition of small amounts of tryptic meat broth or yeast extracts. The active principle in these additions was thought to be related to, but not identical w...

Pigment of Nocardia corallina.

Hydrolysis of ibuprofen nitrile and ibuprofen amide and deracemisation of ibuprofen using Nocardia corallina B-276.

A novel application of whole cells of Nocardia corallina B-276 for the deracemisation of ibuprofen is reported. This microorganism successfully hydrolysed ibuprofen nitrile to ibuprofen amide, and ibuprofen amide to ibuprofen, using a suspension of cells in a potassium phosphate buffer solution (0.1 M, pH = 7.0). These results can be explained by the presence of NHase and amidase enzymes, but t...