Application of Statistical Design to Optimize Culture Medium for Xylanase Production by Bacillus pumilus AB-1

234 Application of Statistical Design to Optimize Culture Medium for Xylanase Production by Bacillus pumilus AB-1 Snehal Ingale, Sonam Kalyani 1 and Urvish Chhaya Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology & Allied Sciences, New Vallabh Vidyanagar-388120, Gujarat, India Natubhai V. Patel College of Pure and Applied Sciences, Vallabh Vidyanagar-388120, Gujarat, India *Corresponding Author E-mail: [email protected] ______________________________________________________________________________ ABSTRACT The objective of the study was to optimize the culture medium and conditions for the production of xylanase from Bacillus pumilis AB-1. Xylanase production was found to increase when wheat bran is used as a lignocellulosic substrate at pH 5.0 and at 37C temperature when moisture ratio was kept 1:01. Further optimization was carried out using Plackett-Burman design and Central-Composite Design. The Plackett-Burman multifactorial design was first employed to screen the important nutrient sources in the medium for xylanase production by Bacillus pumilis AB-1 and subsequent use of the response surface methodology (RSM) was further optimized for xylanase production by Central Composite Design. Wheat bran, pH and Temperature showed greater value of the F-ratio then the F-table (F-ratio > 4.21001) and exerted positive influence on the Xylanase production. The Central Composite Design predicts that the xylanase production is located at the actual values of 1.86 gm% of wheat bran at pH 6.21. After the statistical optimization 5.58 fold increase in the xylanase production was observed over unoptimized medium.