Biochemical changes occurring during growth of Coccidioides immitis in a defined medium.
نویسندگان
چکیده
The nutritional requirements of Coccidioides immitis strain Cash, grown in a medium conzisting of glucose, ammonium lactate, and in-organic salts, were reported by Goldschmidt and Taylor (1958). The present paper considers some of the biochemical changes which take place in this medium during growth. The utilization of glucose, lactate, and ammonia was studied with respect to growth, fragmentation of hyphae, and the accumulation of extracellular peptides and polysaccharide(s) during growth.
منابع مشابه
Nutrition of the Parasitic Phase of Coccidioides Immitis in a Chemically Defined Liquid Medium.
In previous reports (Converse, 1955, 1956, 1957) a chemically defined medium was described which supported the growth of the parasitic phase (spherule) of the dimorphic fungus Coccidioides immitis. Certain physicochemical factors were shown to influence the growth of spherules in this medium. The work to be described here is an extension of the investigation of the nutritional and environmental...
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The chief obstacles to the study of the parasitic or in vivo form (spherule) of Coccidioides immitis grown in synthetic media have been: (1) separating spherules from hyphae, (2) obtaining rupture of the majority of the spherules, and (3) failure of the endospores to separate from the spherule matrix. Growth of spherules in a chemically defined liquid medium composed of glucose, ammo(liberated ...
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Coccidioides immitis, a dimorphic pathogenic fungus, when grown in vitro on solid or in liquid media, usually forms mycelium with septate hyphae which, upon continued incubation, fragment into numerous arthrospores approximately 2 to 4 A in diameter. No definitive studies on the production of arthrospores in chemically defined liquid media have appeared since the work of Roessler et al. (1946),...
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ورودعنوان ژورنال:
- Journal of bacteriology
دوره 75 6 شماره
صفحات -
تاریخ انتشار 1958