Iranian Journal of Basic Medical Sciences
نویسندگان
چکیده
Article type: Original article Objective(s): The utilization of molecular techniques in the epidemiology of tuberculosis have provided an opportunity for using effective markers to trace the transmission of the disease .The purpose of this study was to compare the genetic patterns of Mycobacterium tuberculosis by three methods of RFLP technique. Materials and Methods: In a cross-sectional and prospective study, 95 strains of M. tuberculosis isolates were selected for DNA fingerprinting. Extraction of DNA from Mycobacterium strains and DNA fingerprinting with IS-6110, PGRS and DR probe were performed by standard protocols. Results: Overall, the diversity of RFLP among 95 tuberculosis patients were 48, 50 and 45 on the basis of IS6110, PGRS and DR patterns, respectively. Twenty of these patterns (21.1%) with IS6110-RFLP, twenty-two (23.2%) with PGRS-RFLP and seventeen (17.9%) with DR-RFLP occurred with unique RFLP patterns, whereas the remaining 28 patterns were communal. The risk factors of clustering among tuberculosis patients were age < 45 years, new cases, degree of sputum smear ≥ 2+, and close contact. Conclusion: Our study demonstrated that IS6110-RFLP, PGRS-RFLP and DR-RFLP genotyping could roughly identify similar proportions of clustered (secondary) cases as well as the same risk factors for clustering. Article history: Received: Jun 17, 2014 Accepted: Apr 9, 2014
منابع مشابه
A brief report on Iranian Journal of Basic Medical Sciences in 2013
This editorial deals with the state of the Iranian Journal of Basic Medical Sciences (IJBMS) in 2013. In the previous year, we received 613 manuscripts for publication. After peer-review by expert national and international reviewers, 15 % of submitted papers were accepted for publication. As presented in Figure 1, the rejection rate was around 60%. The publication frequency was doubled by swit...
متن کاملScience production in Iran: The scenario of Iranian medical journals
In Iran, the number of published articles has increased significantly in the basic and applied sciences including medicine and its subspecialties during the recent years. The present study reviewed Iranian science production in medicine, focusing on Iranian medical journals and assessing the current status of Iranian medical journals in several information databases. The study revealed that onl...
متن کاملCitation Analysis of Iranian Journal of Basic Medical Sciences in ISI Web of Knowledge, Scopus, and Google Scholar
OBJECTIVE(S) Citation tracking is an important method to analyze the scientific impact of journal articles and can be done through Scopus (SC), Google Scholar (GS), or ISI web of knowledge (WOS). In the current study, we analyzed the citations to 2011-2012 articles of Iranian Journal of Basic Medical Sciences (IJBMS) in these three resources. MATERIAL AND METHODS The relevant data from SC, GS...
متن کامل