Determination of the sensitivity of a rapid Escherichia coli O157:H7 assay for testing 375-gram composite samples.

نویسندگان

  • W L Tsai
  • C E Miller
  • E R Richter
چکیده

Both 25-g single-size ground beef samples and 375-g composite ground beef samples were tested by a method combining an immunomagnetic separation (IMS) technique with a sandwich enzyme-linked immunosorbent assay (ELISA) system (IMS-ELISA). The results demonstrated that IMS-ELISA could detect the target, Escherichia coli O157:H7, at the level of 10(-1) CFU/g of sample in either the 25- or 375-g sample size.

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عنوان ژورنال:
  • Applied and environmental microbiology

دوره 66 9  شماره 

صفحات  -

تاریخ انتشار 2000