In Vitro Activation of the Contact ( Hageman Factor ) System of Plasma by Heparin and Chondroitin Sulfate
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چکیده
A large number of negatively charged macromolecules, including DNA, glycosaminoglycans. and proteoglycans. were tested as possible activators of the contact (Hageman factor) system in vitro. Activation was assessed by conversion of prekallikrein to kallikrein. as determined by amidolytic assay and by cleavage of 12 1-Hageman factor into 52.000and 28.000-dalton fragments. Of particular interest to these studies, heparin proteoglycan and glycosaminoglycan from rat peritoneal mast cells. and squid chondroitin sulfate E, which is representative of the glycosaminoglycan from cultured mouse bone marrow derived mast cells. induced the reciprocal activation between Hageman factor and prekallikrein. In addition, naturally occuring heparin glycosaminoglycans from pig mucosa, bovine lung. and rat mast cells also induced activation. In contrast. native connective tissue matrix glycosaminoglycans and proteoglycans from several sources were inactive, although when
منابع مشابه
In vitro activation of the contact (Hageman factor) system of plasma by heparin and chondroitin sulfate E.
A large number of negatively charged macromolecules, including DNA, glycosaminoglycans, and proteoglycans, were tested as possible activators of the contact (Hageman factor) system in vitro. Activation was assessed by conversion of prekallikrein to kallikrein, as determined by amidolytic assay and by cleavage of 125I-Hageman factor into 52,000- and 28,000-dalton fragments. Of particular interes...
متن کاملBeyond health care--socioeconomic status and health.
n engl j med 358;23 www.nejm.org june 5, 2008 2509 tivation of the contact system. N Engl J Med 2008;358:2457 67. Petitou M, Casu B, Lindahl U. 19761983, A critical period in the history of heparin: the discovery of the antithrombin bind ing site. Biochimie 2003;85:839. Hojima Y, Cochrane CG, Wiggins RC, Austen KF, Stevens RL. In vitro activation of the contact (Hageman factor) system of pl...
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Purified radiolabeled rabbit Hageman factor, prekallikrein, and high molecular weight kininogen were used to examine Hageman factor system molecular dynamics after the intravenous injection of heparin-like dextran sulfate polymer in the rabbit. Hageman factor system proteins rapidly disappeared from the circulation following dextran sulfate injection, as measured by radial immunodiffusion, by k...
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