Functional Glycomic Analysis of Human Milk Glycans Reveals Presence of Virus Receptors and Embryonic Stem Cell Biomarkers
نویسندگان
چکیده
Department of Biochemistry and the Glycomics Center; Emory University School of Medicine; Atlanta, GA 30322; Department of Microbiology and Immunology; Emory University School of Medicine; Atlanta, GA 30322; Department of Biochemistry & Molecular Biology; University of Oklahoma Health Sciences Center; Oklahoma City, OK 73126; Department of Biochemistry & Molecular Biology; University of Florida, Gainesville, FL 32607; Department of Laboratory Medicine, Yale School of Medicine, New Haven, CT 06519.
منابع مشابه
Functional glycomic analysis of human milk glycans reveals the presence of virus receptors and embryonic stem cell biomarkers.
Human milk contains a large diversity of free glycans beyond lactose, but their functions are not well understood. To explore their functional recognition, here we describe a shotgun glycan microarray prepared from isolated human milk glycans (HMGs), and our studies on their recognition by viruses, antibodies, and glycan-binding proteins (GBPs), including lectins. The total neutral and sialylat...
متن کاملTotal cellular glycomics allows characterizing cells and streamlining the discovery process for cellular biomarkers.
Although many of the frequently used pluripotency biomarkers are glycoconjugates, a glycoconjugate-based exploration of novel cellular biomarkers has proven difficult due to technical difficulties. This study reports a unique approach for the systematic overview of all major classes of oligosaccharides in the cellular glycome. The proposed method enabled mass spectrometry-based structurally int...
متن کاملThe characterization of CD marker profile of breast milk-derived stem cell
Background The mammary gland in humans is a dynamic organ that undergoes significant developmental changes during pregnancy, lactation, and involution. Stem cells derived from human breast milk possess the adult stem cell-like characteristics such as self-renewal, proliferative and differentiate potential. This source of stem cells avoids invasive procedures and the ethical controversy of emb...
متن کاملRecent Advances in Cellular Glycomic Analyses
A large variety of glycans is intricately located on the cell surface, and the overall profile (the glycome, given the entire repertoire of glycoconjugate-associated sugars in cells and tissues) is believed to be crucial for the diverse roles of glycans, which are mediated by specific interactions that control cell-cell adhesion, immune response, microbial pathogenesis and other cellular events...
متن کاملA Lectin-Based Glycomic Approach to Identify Characteristic Features of Xenopus Embryogenesis
Cell surface glycans show dynamic changes during cell differentiation. Several glycans are useful biomarkers of tumors, stem cells, and embryogenesis. Glycomic studies have been performed using liquid chromatography and mass spectrometry, which are powerful tools for glycan structural analysis but are difficult to use for small sample sizes. Recently, a lectin microarray system was developed fo...
متن کامل